3HQB
Crystal structure of human desarg-C5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1991-06-19 |
Detector | SIEMENS-NICOLET X100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 54.720, 54.720, 96.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.799 - 3.299 |
R-factor | 0.2191 |
Rwork | 0.210 |
R-free | 0.30690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hqa |
RMSD bond length | 0.012 |
RMSD bond angle | 1.516 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.300 |
High resolution limit [Å] | 3.299 |
Number of reflections | 2707 |
Completeness [%] | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |