3HPW
CcdB dimer in complex with one C-terminal CcdA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8423 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.357, 38.038, 69.651 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 15.785 - 1.452 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CcdB2 dimer PDB entry 2VUB chain A B |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.162 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.780 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.043 | 0.107 |
| Number of reflections | 36600 | |
| <I/σ(I)> | 18.3 | 7.8 |
| Completeness [%] | 95.9 | 98.2 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 22% PEG4000, 0.1M Tris-HCl pH7.4, 0.2M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
