3HPM
Oxidized dimeric PICK1 PDZ C46G mutant in complex with the carboxyl tail peptide of GluR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.450, 54.000, 92.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.620 - 2.800 |
| R-factor | 0.24807 |
| Rwork | 0.243 |
| R-free | 0.29504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pku |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.620 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.064 | 0.377 |
| Number of reflections | 4797 | |
| <I/σ(I)> | 7.6 | 2 |
| Completeness [%] | 96.8 | 96.8 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% (w/v) PEG4000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
