3HPJ
Human Class I MHC HLA-A2 in complex with the WT-1 (126-134) peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-26 |
Detector | CUSTOM-MADE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.595, 86.780, 79.116 |
Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tvb |
RMSD bond length | 0.018 |
RMSD bond angle | 1.741 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.086 | 0.468 |
Number of reflections | 56716 | |
<I/σ(I)> | 14.106 | 2 |
Completeness [%] | 97.6 | 86.2 |
Redundancy | 3.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | PEG3350 24%, MES 0.025M, NaCl 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |