3HPJ
Human Class I MHC HLA-A2 in complex with the WT-1 (126-134) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-26 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.595, 86.780, 79.116 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.741 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.468 |
| Number of reflections | 56716 | |
| <I/σ(I)> | 14.106 | 2 |
| Completeness [%] | 97.6 | 86.2 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | PEG3350 24%, MES 0.025M, NaCl 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
