3HPC

Crystal structure of SNX5-PX domain in P21 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-15
Detector	RIGAKU SATURN 944
Spacegroup name	P 1 21 1
Unit cell lengths	43.598, 41.424, 47.227
Unit cell angles	90.00, 92.98, 90.00

Refinement procedure

Resolution	31.120 - 1.470
R-factor	0.187
Rwork	0.184
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hpb
RMSD bond length	0.013
RMSD bond angle	1.296
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0044)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.120	1.520
High resolution limit [Å]	1.470	1.470
Rmerge	0.043	0.325
Number of reflections	28322
<I/σ(I)>	20.1	1.4
Completeness [%]	98.3	90.8
Redundancy	4.13	2.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277	The protein solution in the final superdex-75 buffer (20 mM Tris.HCl, 100 mM NaCl, 0.02% NaN3, pH 8.0) was concentrated to 8 mg/ml and crystallization were obtained using an optimized crystallization condition of 8ul of protein vs 1ul of reservoir solution (0.2M NH4CH3COO, 0.1M Sodium cacodylate tryhydrate pH 6.5, 30% PEG 4000), VAPOR DIFFUSION, SITTING DROP, temperature 277K