3HPC
Crystal structure of SNX5-PX domain in P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-15 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.598, 41.424, 47.227 |
Unit cell angles | 90.00, 92.98, 90.00 |
Refinement procedure
Resolution | 31.120 - 1.470 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hpb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.296 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.120 | 1.520 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.043 | 0.325 |
Number of reflections | 28322 | |
<I/σ(I)> | 20.1 | 1.4 |
Completeness [%] | 98.3 | 90.8 |
Redundancy | 4.13 | 2.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | The protein solution in the final superdex-75 buffer (20 mM Tris.HCl, 100 mM NaCl, 0.02% NaN3, pH 8.0) was concentrated to 8 mg/ml and crystallization were obtained using an optimized crystallization condition of 8ul of protein vs 1ul of reservoir solution (0.2M NH4CH3COO, 0.1M Sodium cacodylate tryhydrate pH 6.5, 30% PEG 4000), VAPOR DIFFUSION, SITTING DROP, temperature 277K |