3HPB
Crystal structure of SNX5-PX domain in P212121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-17 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.957, 75.874, 77.512 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.780 - 2.190 |
R-factor | 0.24 |
Rwork | 0.236 |
R-free | 0.28400 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.284 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SnB |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.780 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.033 | 0.256 |
Number of reflections | 12680 | |
<I/σ(I)> | 28.1 | 3.5 |
Completeness [%] | 93.4 | 62.3 |
Redundancy | 4.18 | 2.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | the protein solution in the final superdex-75 buffer (20 mM Tris.HCl, 100 mM NaCl, 0.02% NaN3, pH 8.0) was concentrated to 8 mg/ml. The Se-Met derivative and the 2.19 A crystals were obtained using an optimized initial crystallization condition of 8 ul of protein vs 1 ul of reservoir solution (0.2M (NH4)2SO4, 0.1M Sodium cacodylate tryhydrate pH 6.5, 30% PEG 8000) at 4C by sitting drop vapor diffusion, VAPOR DIFFUSION, SITTING DROP, temperature 277K | |
1 | 8 | 277 | the protein solution in the final superdex-75 buffer (20 mM Tris.HCl, 100 mM NaCl, 0.02% NaN3, pH 8.0) was concentrated to 8 mg/ml. The Se-Met derivative and the 2.19 A crystals were obtained using an optimized initial crystallization condition of 8 ul of protein vs 1 ul of reservoir solution (0.2M (NH4)2SO4, 0.1M Sodium cacodylate tryhydrate pH 6.5, 30% PEG 8000) at 4C by sitting drop vapor diffusion, VAPOR DIFFUSION, SITTING DROP, temperature 277K |