3HP3
Crystal structure of CXCL12
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0400 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.834, 117.468, 134.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.180 - 2.200 |
R-factor | 0.2294 |
Rwork | 0.228 |
R-free | 0.27995 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.229 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 39406 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M Tris-HCl pH 8.5, 20% Jeffamine M-600, VAPOR DIFFUSION, HANGING DROP, temperature 300K |