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3HOZ

Complete RNA polymerase II elongation complex IV with a T-U mismatch and a frayed RNA 3'-guanine

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2008-03-17
DetectorPSI PILATUS 6M
Wavelength(s)0.9188
Spacegroup nameC 2 2 21
Unit cell lengths221.427, 393.750, 281.818
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 3.650
Rwork0.210
R-free0.25300
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3hou
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareCNS (1.2)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]3.650
Number of reflections135977
<I/σ(I)>22
Completeness [%]99.9
Redundancy7.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP729320mM ammonium acetate, 300mM SODIUM ACETATE, 50mM Hepes pH 7.0, 4-6% PEG 6000, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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