3HOZ

Complete RNA polymerase II elongation complex IV with a T-U mismatch and a frayed RNA 3'-guanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-03-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.9188
Spacegroup name	C 2 2 21
Unit cell lengths	221.427, 393.750, 281.818
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.650
Rwork	0.210
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hou
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.650
Number of reflections	135977
<I/σ(I)>	22
Completeness [%]	99.9
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20mM ammonium acetate, 300mM SODIUM ACETATE, 50mM Hepes pH 7.0, 4-6% PEG 6000, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K