3HOP
Structure of the actin-binding domain of human filamin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.850, 71.220, 158.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.570 - 2.300 |
R-factor | 0.25236 |
Rwork | 0.250 |
R-free | 0.28820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wku |
RMSD bond length | 0.018 |
RMSD bond angle | 1.651 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.900 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.530 |
Number of reflections | 28211 | |
<I/σ(I)> | 9.4 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 4000, 0.2M lithium sulfate, 0.1M Tris HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |