3HOH
RIBONUCLEASE T1 (THR93GLN MUTANT) COMPLEXED WITH 2'GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.360, 60.550, 100.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.950 |
R-factor | 0.167 |
Rwork | 0.167 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rga |
RMSD bond length | 0.006 |
RMSD bond angle | 1.505 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 26956 | |
<I/σ(I)> | 17.9 | 5.3 |
Completeness [%] | 98.8 | 97.4 |
Redundancy | 11.84 | 3.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.2 | 25 MM NAAC PH 4.2 6.25 MM CACL2 42.5 % MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 1 | 25 (mM) | ||
3 | 1 | 1 | 2'GMP | 2.8 (mM) | |
4 | 1 | 1 | 6.25 (mM) | ||
5 | 1 | 1 | MPD | 42.5 (%) |