3HO7
Crystal structure of OxyR from Porphyromonas gingivalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 55.819, 55.795, 56.711 |
Unit cell angles | 110.63, 102.82, 114.63 |
Refinement procedure
Resolution | 26.940 - 1.580 |
R-factor | 0.22028 |
Rwork | 0.219 |
R-free | 0.24456 |
Structure solution method | MIR |
RMSD bond length | 0.003 |
RMSD bond angle | 0.648 |
Data reduction software | d*TREK |
Data scaling software | CrystalClear |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.940 | |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.032 | 0.032 |
Number of reflections | 59646 | |
Completeness [%] | 86.2 | 86.2 |
Redundancy | 3.92 | 3.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 12% PEG 8K, 8% EG, 0.1M HEPES BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 12% PEG 8K, 8% EG, 0.1M HEPES BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 12% PEG 8K, 8% EG, 0.1M HEPES BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 12% PEG 8K, 8% EG, 0.1M HEPES BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |