3HNZ

Structure of E. coli FabF(C163A) in Complex with Platensimycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	74.665, 74.665, 146.274
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.930 - 2.750
R-factor	0.182
Rwork	0.178
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.431
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.930	50.000	2.850
High resolution limit [Å]	2.750	5.920	2.750
Rmerge	0.069	0.028	0.419
Number of reflections	12790
<I/σ(I)>	18.917
Completeness [%]	99.8	98.5	100
Redundancy	3.5	3.2	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1