3HNX
Crystal structure of a designed Cyanovirin-N homolog lectin; LKAMG in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-17 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.506, 46.204, 50.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.260 - 1.370 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hnu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.260 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.039 | 0.337 |
| Number of reflections | 23394 | |
| <I/σ(I)> | 40.7 | 2.7 |
| Completeness [%] | 97.7 | 77.3 |
| Redundancy | 13.32 | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 50 mg/ml protein concentration in 0.2 M Li2SO4, 0.1 M Tris-HCl (pH 8.5), and 30% PEG 4000 at protein to mother liquor ratio of 8 to 1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
