3HNK
Crystal structure of the dimeric assembly of the cyt cb562 variant RIDC-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.318, 55.484, 72.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.750 - 2.100 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.994 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.860 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.249 |
| Number of reflections | 12085 | |
| <I/σ(I)> | 13.4 | 4.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 3.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 26% PEG 2000, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
