3HMY
Crystal structure of HCR/T complexed with GT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.960, 72.690, 119.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.200 - 2.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1fv2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39755 | |
| <I/σ(I)> | 24.8 | 3.6 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | Bis-Tis propane buffer pH 7.0, 25% PEG2K and 300 mM Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
