3HM6
Crystal structure of the cytoplasmic domain of human plexin B1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | CCD |
Collection date | 2007-09-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9999 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.346, 74.346, 214.398 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.083 - 2.402 |
R-factor | 0.228 |
Rwork | 0.227 |
R-free | 0.26200 |
Structure solution method | SAD |
Starting model (for MR) | For phasing native crystals were incubated over-night in an artificial mother liquor containing 0.001 M thimerosal. |
RMSD bond length | 0.013 |
RMSD bond angle | 1.213 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmerge | 0.045 | 0.019 | 0.577 |
Number of reflections | 27657 | ||
<I/σ(I)> | 15 | ||
Completeness [%] | 99.9 | 99.2 | 99.9 |
Redundancy | 9.8 | 9.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 16% peg-2000 mme, 0.1M TRIS, 0.2M TMAO, pH 8, vapor diffusion, hanging drop, temperature 291K |