3HM5

SANT domain of human DNA methyltransferase 1 associated protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-04-06
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.91987
Spacegroup name	P 61 2 2
Unit cell lengths	36.523, 36.523, 230.506
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.905 - 1.800
R-factor	0.225
Rwork	0.223
R-free	0.27500
Structure solution method	SIRAS
RMSD bond length	0.015
RMSD bond angle	1.455
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.079	0.042	0.470
Number of reflections	9198
<I/σ(I)>	44.467
Completeness [%]	96.8	99.3	75.6
Redundancy	17	16.7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	20% PEG3350, 0.2M calcium chloride, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7	291	20% PEG3350, 0.2M calcium chloride, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7	291	20% PEG3350, 0.2M calcium chloride, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 291K