3HLS
Crystal structure of the signaling helix coiled-coil doimain of the BETA-1 subunit of the soluble guanylyl cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.039, 65.814, 98.626 |
| Unit cell angles | 90.00, 129.95, 90.00 |
Refinement procedure
| Resolution | 39.810 - 2.150 |
| R-factor | 0.21568 |
| Rwork | 0.213 |
| R-free | 0.26113 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.810 |
| High resolution limit [Å] | 2.150 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 99.2 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1 M Bis-Tris pH 7.0, 0.7M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
