3HLM
Crystal Structure of Mouse Mitochondrial Aspartate Aminotransferase/Kynurenine Aminotransferase IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 284.322, 76.792, 87.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.500 |
| R-factor | 0.18354 |
| Rwork | 0.181 |
| R-free | 0.23429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9aat |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 67188 | |
| Redundancy | 12.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 20% PEG 4000, 100 mM Ammonium Sulphate, 6% Glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
