3HL1
CRYSTAL STRUCTURE OF A FERRITIN LIKE PROTEIN (CC_0557) FROM CAULOBACTER VIBRIOIDES AT 1.95 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97876,0.97828 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.633, 44.017, 119.381 |
| Unit cell angles | 90.00, 96.17, 90.00 |
Refinement procedure
| Resolution | 29.424 - 1.950 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.424 | 29.424 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.190 | 1.950 |
| Rmerge | 0.039 | 0.019 | 0.501 |
| Number of reflections | 41306 | 7752 | 7676 |
| <I/σ(I)> | 12.61 | 35.8 | 1.7 |
| Completeness [%] | 96.9 | 95.3 | 94.5 |
| Redundancy | 3.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.93 | 293 | 17.5000% polyethylene glycol 6000, 0.1M citric acid pH 4.93, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K |
