3HKX
Crystal structure analysis of an amidase from Nesterenkonia sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2009-01-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.999, 115.639, 65.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.500 - 1.660 |
| R-factor | 0.18713 |
| Rwork | 0.186 |
| R-free | 0.21433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f89 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.674 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.511 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.046 | 0.360 |
| Number of reflections | 34232 | |
| <I/σ(I)> | 15.6 | 4.2 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 7.03 | 6.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M Sodium acetate trihydrate and 2M ammonium sulphate, pH 4.6, vapor diffusion, hanging drop, temperature 293K |
