3HKL
Crystal Structure of the Frizzled-like Cysteine-rich Domain of MuSK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.592, 44.422, 69.530 |
| Unit cell angles | 90.00, 108.27, 90.00 |
Refinement procedure
| Resolution | 34.470 - 2.100 |
| R-factor | 0.22822 |
| Rwork | 0.226 |
| R-free | 0.26433 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.470 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 22050 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 96.9 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 5% PEG 4000, 0.2M SODIUM ACETATE, 0.1M TRIS PH 8.5, 5% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
