3HJZ
The structure of an aldolase from Prochlorococcus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.755, 80.376, 97.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.190 - 1.900 |
| R-factor | 0.15837 |
| Rwork | 0.156 |
| R-free | 0.20255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | produced by inputing the protein sequence into Swiss-Model. Model based on the structure of 1F05 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.190 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.372 |
| Number of reflections | 26276 | |
| <I/σ(I)> | 22.25 | 5.7 |
| Completeness [%] | 96.1 | 71.1 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Hepes 7.5, 20% PEG 10K and 1/20 TEV. 10% ethylene glycol, then Paratone-N oil used as cryoprotectant , VAPOR DIFFUSION, SITTING DROP, temperature 294K |
