3HJT
Structure of laminin binding protein (Lmb) of Streptococcus agalactiae A bifunctional protein with adhesin and metal transporting activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-10-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.641, 70.411, 74.971 |
Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cx3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | AUTOMAR |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.054 | 0.274 |
Number of reflections | 19066 | |
<I/σ(I)> | 5.6 | 1.4 |
Completeness [%] | 98.7 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 293 | 30-35% (w/v) 30% PEG 2000 monoethyl ether, 0.1mM sodium citrate pH5.0, 5mM NiCl2 and 10% ethylene glycol, VAPOR DIFFUSION, temperature 293K |