3HJL
The structure of full-length FliG from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.997, 65.840, 67.791 |
Unit cell angles | 90.00, 110.99, 90.00 |
Refinement procedure
Resolution | 19.952 - 2.400 |
R-factor | 0.219 |
Rwork | 0.216 |
R-free | 0.27000 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.065 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX (& SHARP) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.952 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.051 | 0.322 |
Number of reflections | 19157 | |
<I/σ(I)> | 10.4 | 4.1 |
Completeness [%] | 98.7 | 99.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.75 | 293 | 45% 1,4 butanediol, 0.1M Tris-Acetate, pH 8.75, VAPOR DIFFUSION, SITTING DROP, temperature 293K |