3HJL
The structure of full-length FliG from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.997, 65.840, 67.791 |
| Unit cell angles | 90.00, 110.99, 90.00 |
Refinement procedure
| Resolution | 19.952 - 2.400 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (& SHARP) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.952 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.051 | 0.322 |
| Number of reflections | 19157 | |
| <I/σ(I)> | 10.4 | 4.1 |
| Completeness [%] | 98.7 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.75 | 293 | 45% 1,4 butanediol, 0.1M Tris-Acetate, pH 8.75, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
