3HJH
A rigid N-terminal clamp restrains the motor domains of the bacterial transcription-repair coupling factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.062, 157.650, 35.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.030 - 1.950 |
| R-factor | 0.21282 |
| Rwork | 0.210 |
| R-free | 0.25731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eyq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.060 | 1.990 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.055 | 0.649 |
| Number of reflections | 36838 | |
| <I/σ(I)> | 27.9 | 2.6 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 6.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 8% PEG 4000, 0.01 M CO(II)CL2, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
