3HJE
Crystal structure of sulfolobus tokodaii hypothetical maltooligosyl trehalose synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-11 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.992, 86.100, 70.130 |
| Unit cell angles | 90.00, 95.43, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.900 |
| R-factor | 0.17245 |
| Rwork | 0.171 |
| R-free | 0.20606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iv8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.079 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.843 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 59664 | |
| Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.89 | 293 | 0.2M Potassium sodium tartrate tetrahydrate, 20%(w/v) PEG3350, pH 5.89, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
