3HJ2
Crystal structure of covalent dimer of HNP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.070, 49.370, 24.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.20059 |
| Rwork | 0.199 |
| R-free | 0.23578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.680 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.065 | 0.114 |
| Number of reflections | 11611 | |
| <I/σ(I)> | 22.5 | 17.8 |
| Completeness [%] | 96.3 | 100 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M Na-citrate (pH 5.6), 0.2 M NH4-acetate, 30% (v/v) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
