3HJ1
Minor Editosome-Associated TUTase 1 with bound UTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-14 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.873, 101.999, 66.124 |
Unit cell angles | 90.00, 111.65, 90.00 |
Refinement procedure
Resolution | 31.050 - 1.950 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.401 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.127 | 0.490 |
Number of reflections | 54366 | |
<I/σ(I)> | 12.528 | |
Completeness [%] | 96.4 | 83.5 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 291 | 0.2M lithium acetate, 25% PEG 3350, 0.5mM UTP, pH 8.0, vapor diffusion, temperature 291K |