3HIY
Minor Editosome-Associated TUTase 1 with bound UTP and Mg
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97876, 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.093, 103.024, 63.041 |
| Unit cell angles | 90.00, 111.01, 90.00 |
Refinement procedure
| Resolution | 33.710 - 2.300 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.092 | 0.045 | 0.480 |
| Number of reflections | 67571 | ||
| <I/σ(I)> | 21.66 | ||
| Completeness [%] | 99.6 | 99.7 | 98.7 |
| Redundancy | 3.8 | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 291 | 0.2M lithium acetate, 25% PEG 3350, 0.5mM UTP, 10mM MgCl2, pH 8.0, vapor diffusion, temperature 291K |
