3HIU
The crystal structure of protein (XCC3681) from Xanthomonas campestris pv. campestris str. ATCC 33913
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 102.592, 56.843, 53.990 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 49.720 - 1.850 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.25700 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.951 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.108 | 0.807 |
Number of reflections | 52443 | |
<I/σ(I)> | 18.9 | 1.14 |
Completeness [%] | 98.9 | 97.9 |
Redundancy | 3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 293 | 0.1M K Thiocyanate, 30% PEG2KMME, VAPOR DIFFUSION, SITTING DROP, temperature 293K |