3HIL
SAM Domain of Human Ephrin Type-A Receptor 1 (EphA1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91700 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.286, 52.286, 81.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.21163 |
| Rwork | 0.208 |
| R-free | 0.27942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qkq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.393 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 9138 | |
| <I/σ(I)> | 43 | 3.64 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.1 | 22% PEG 3350, 0.3 M MG(NO4)2. PRIOR TO SETTING UP CRYSTALLIZATION PLATES, CHYMOTRYPSIN WAS ADDED TO THE PROTEIN SAMPLE AT A FINAL CONCENTRATION OF 0.57 MICROMOLAR., pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.1K |
