3HIE

Structure of the membrane-binding domain of the Sec3 subunit of the Exocyst complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-20
Detector	MAR CCD 165 mm
Spacegroup name	P 1
Unit cell lengths	40.697, 68.486, 68.036
Unit cell angles	89.90, 82.17, 71.79

Refinement procedure

Resolution	38.740 - 2.000
R-factor	0.204
Rwork	0.202
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.025
RMSD bond angle	21.689
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.520
Number of reflections	43622
<I/σ(I)>	24.6	1.5
Completeness [%]	93.6	62.7
Redundancy	10.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	0.1M Bicine, pH9.5, 14% PEG 5000 MME, 1mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1		7.5	293	0.1M Bicine, pH9.5, 14% PEG 5000 MME, 1mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1		7.5	293	0.1M Bicine, pH9.5, 14% PEG 5000 MME, 1mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1		7.5	293	0.1M Bicine, pH9.5, 14% PEG 5000 MME, 1mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K