3HID
Crystal structure of adenylosuccinate synthetase from Yersinia pestis CO92
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.903, 68.903, 199.166 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.700 - 1.600 |
R-factor | 0.16213 |
Rwork | 0.160 |
R-free | 0.19756 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.661 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.700 | 1.642 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.092 | 0.394 |
Number of reflections | 60809 | |
<I/σ(I)> | 35.46 | 3.2 |
Completeness [%] | 99.7 | 98.24 |
Redundancy | 12.7 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M Ammonium sulfate, 30% w/v PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |