3HID
Crystal structure of adenylosuccinate synthetase from Yersinia pestis CO92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.903, 68.903, 199.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.700 - 1.600 |
| R-factor | 0.16213 |
| Rwork | 0.160 |
| R-free | 0.19756 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.661 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.700 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.092 | 0.394 |
| Number of reflections | 60809 | |
| <I/σ(I)> | 35.46 | 3.2 |
| Completeness [%] | 99.7 | 98.24 |
| Redundancy | 12.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2M Ammonium sulfate, 30% w/v PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
