3HIB
Crystal structure of the second Sec63 domain of yeast Brr2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-04 |
Detector | NOIR-1 |
Wavelength(s) | 0.9790, 0.9789, 0.9640 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.153, 75.678, 83.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.25700 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.674 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.084 | 0.414 |
Number of reflections | 45853 | |
<I/σ(I)> | 10.7 | 3.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M Sodium citrate, 17% PEG 8000, 0.2M NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |