3HI2
Structure of the N-terminal domain of the E. coli antitoxin MqsA (YgiT/b3021) in complex with the E. coli toxin MqsR (YgiU/b3022)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-10 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 63.234, 63.234, 194.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.640 - 2.000 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.338 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 26665 | |
<I/σ(I)> | 35.99 | 8.56 |
Completeness [%] | 95.8 | 98 |
Redundancy | 12.5 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 277 | 0.1M Bis Tris, 25% PEG 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K | |
1 | 5.6 | 277 | 0.1M Bis Tris, 25% PEG 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K | |
1 | 5.6 | 277 | 0.1M Bis Tris, 25% PEG 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K | |
1 | 5.6 | 277 | 0.1M Bis Tris, 25% PEG 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |