3HHP
Malate dehydrogenase open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.026, 51.972, 168.980 |
Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
Resolution | 60.970 - 1.450 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.065 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | BALBES |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.971 | 1.490 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.056 | |
Number of reflections | 215865 | |
<I/σ(I)> | 1.5 | |
Completeness [%] | 98.4 | 94.8 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100 mM ADA, 0.2 M sodium acetate, 30% PEG MME 5000, 5% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |