3HHL
Crystal structure of methylated RPA0582 protein
Experimental procedure
Experimental method | MOLECULAR REPLACEMENT |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-31 |
Detector | SBC-3 |
Wavelength(s) | 0.9787 |
Spacegroup name | H 3 |
Unit cell lengths | 193.012, 193.012, 117.965 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.170 - 2.650 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dca |
RMSD bond length | 0.017 |
RMSD bond angle | 1.584 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.670 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.090 | 0.596 |
Number of reflections | 47581 | |
<I/σ(I)> | 24.26 | 2.3 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M AMMONIUM SULPHATE, 25% PEG3350, 0.1M HEPES pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |