3HH8
Crystal Structure and metal binding properties of the lipoprotein MtsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2006-06-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.260, 50.077, 157.617 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.410 - 1.870 |
R-factor | 0.17338 |
Rwork | 0.172 |
R-free | 0.19862 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.466 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.900 | 1.960 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.065 | 0.320 |
Number of reflections | 25230 | |
<I/σ(I)> | 14.8 | 3.5 |
Completeness [%] | 96.1 | 96 |
Redundancy | 3.9 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 27% PEGMME 2K, 0.1M MgCl2, 10mM HEPES/KOH, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |