3HGV
Structure of Phenazine Antibiotic Biosynthesis Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-27 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.836, 110.143, 112.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.340 - 2.300 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 | |
| Number of reflections | 38679 | |
| <I/σ(I)> | 45.3 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 5% Tacsimate, 0.1M HEPES, 8-12% PEG MME 5000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
