3HGT
Structural and functional studies of the yeast class II Hda1 HDAC complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 103 |
Detector technology | PIXEL |
Collection date | 2008-09-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0007 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.180, 116.570, 55.190 |
Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
Resolution | 19.983 - 2.200 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 33474 | |
Completeness [%] | 95.4 | 88.7 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |