3HGS
Crystal structure of tomato OPR3 in complex with pHB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.610, 89.500, 80.680 |
Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.212 |
Rwork | 0.206 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.081 | 0.389 |
Number of reflections | 49387 | |
<I/σ(I)> | 10.6 | 2 |
Completeness [%] | 93.9 | 89.4 |
Redundancy | 2.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM MES/Tris, 15% PEG8000, 50 mM ammonium sulfate, pH 7.5, vapor diffusion, sitting drop, temperature 298K |