3HGR
Crystal structure of tomato OPR1 in complex with pHB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.440, 71.990, 72.040 |
| Unit cell angles | 63.09, 84.45, 78.06 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.203 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 | 0.200 |
| Number of reflections | 40037 | |
| <I/σ(I)> | 13.4 | 2.7 |
| Completeness [%] | 97.1 | 80.1 |
| Redundancy | 1.93 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM Tris/HCl, 2% PEG400, 1.45 M ammonium sulfate, pH 7.5, vapor diffusion, sitting drop, temperature 298K |
