3HGO
Crystal structure of the F74Y/H244Y OPR3 double mutant from tomato
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.138, 92.856, 89.624 |
| Unit cell angles | 90.00, 98.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.209 |
| Rwork | 0.200 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.114 | 0.411 |
| Number of reflections | 32400 | |
| <I/σ(I)> | 9.055 | 2.1 |
| Completeness [%] | 91.6 | 89.9 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 1 M (NH4)2HPO4, 0.1 M sodium acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
