3HGM
Universal Stress Protein TeaD from the TRAP transporter TeaABC of Halomonas elongata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 74 |
Detector technology | CCD |
Collection date | 2008-12-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9765 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.050, 74.650, 97.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Poly alanine model of pdb entry 2Z09 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.075 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | 0.734 |
Number of reflections | 42968 | |
<I/σ(I)> | 2.21 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 4.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 100mM Tris pH 7.5, 200mM (NH4)2SO4, 15% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |