3HGA
Crystal Structure of 4-Se-Uridine Derivatized RNA
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 30.255, 34.079, 28.931 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.470 - 1.300 |
R-factor | 0.19414 |
Rwork | 0.192 |
R-free | 0.24301 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.952 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.047 | 0.185 |
Number of reflections | 3773 | |
<I/σ(I)> | 31.605 | |
Completeness [%] | 96.6 | 100 |
Redundancy | 6.8 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | MPD,sodium cacodylate,spermine tetra-HCl, vapor diffusion, hanging drop, temperature 298K |