3HG6
Crystal Structure of the Recombinant Onconase from Rana pipiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.90750 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.516, 39.799, 68.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.700 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.221 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.700 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.128 | 0.498 |
| Number of reflections | 9807 | |
| <I/σ(I)> | 15.403 | 3.84 |
| Completeness [%] | 95.5 | 83.6 |
| Redundancy | 12.8 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | capillary contradiffusion | 4 | 288 | 4 M Ammonium sulphate and 0.1 M sodium acetate, capillary contradiffusion, temperature 288K |
