3HG5
Human alpha-galactosidase catalytic mechanism 4. Product bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.813, 90.813, 217.166 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.670 - 2.300 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.22700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | human alpha-galactosidase |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.175 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.989 |
| Number of reflections | 47076 | |
| <I/σ(I)> | 21.368 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG4000, Ammonium Sulfate, Sodium Acetate, pH 4.6, vapor diffusion, hanging drop, temperature 293K |
