3HFX
Crystal structure of carnitine transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-06-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0039 |
Spacegroup name | P 63 |
Unit cell lengths | 134.210, 134.210, 85.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.150 |
Rwork | 0.262 |
R-free | 0.28100 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.470 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.042 | 3.320 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.070 | 0.391 |
Total number of observations | 109568 | |
Number of reflections | 29078 | |
<I/σ(I)> | 17.5 | 4.4 |
Completeness [%] | 98.2 | 98 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES-NAOH (PH7.5), 22-26% PEG 400, 0.1M NaCl, 18mM n-octyl-D-glucopyranoside (-OG), 5mM L-Carnitine, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K |