3HFR
Crystal structure of glutamate racemase from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-18 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.651, 66.651, 257.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.19109 |
Rwork | 0.188 |
R-free | 0.24411 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.222 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 (SOLVE/RESOLVE) |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.105 | 0.606 |
Number of reflections | 26802 | |
<I/σ(I)> | 37.25 | 4.5 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 17.6 | 15.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Jeffamine ED-2001 pH 7.0, 0.1M Hepes pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |